Peptide Nile is out of Queen Olive (Manhattan Cafe), who won four races. He is a half-brother to Hasedon (Maurice), a Listed-placed winner.

Second dam Olive Branch (Machiavellian) has Queen Olive as her only winner and is a half-sister to four winners.

Third dam Sopranino (Theatrical) was a winner.

The fourth dam My Darling One (Exclusive Native), a Grade 1 winner in the US, produced seven other winners. They are headed by Heart Lake (Nureyev), winner of the Gr.3 Concorde Stakes, placed at Group 2 level in Hong Kong and later a sire, and Darling Flame (Capote), a winner placed in the Gr.3 Cherry Hinton Stakes. Darling Flame is the dam of the Listed winner Bezrin and the grandam of Ten Sovereigns, a dual Group 1 winner and sire, Shamal Wind, a Group 1 winner in Australia, and Al Thakhira, winner of the Gr.2 Rockfel Stakes.

Queen Olive has a 3-year-old filly by Rey de Oro and a yearling colt by Sinister Minister, and she is due to Drefong.