Pauline's Pearl is out of Hot Dixie Chick (Dixie Union), who won the Grade 1 Spinaway S. in 2009 and is a half-sister to the sire Always Dreaming (Bodemeister), winner of the 2017 Grade 1 Kentucky Derby, and to Positive Spirit (Pioneerof the Nile), winner of the Grade 2 Demoiselle S. Pauline's Pearl is a half-sister to Union Jackson (Curlin), a dual stakes winner placed in the Grade 3 Aristides S., and to the winners Marcy Darcy (Smart Strike) and Marsalis (Curlin).

Second dam Above Perfection (In Excess) won the Grade 3 Las Flores Handicap. She is a half-sister to the stakes winner Made To Perfection (Bolger) and to Perfectly Perfect (Oasis Dream), dam of the stakes winners Twenty Hawks and Epitome Of A Lady.

Third dam Something Perfect (Somethingfabulous) was unraced.

Hot Dixie Chick has a 3-year-old, Marsalis (Curlin), and produced a colt by Constitution last season. She was bred to Tapit and Quality Road for 2022.