Twain is the sixth winner for Wading (Montjeu), who won the Gr.2 Rockfel S. at the end of a short career of three starts. Her winners include Just Wonderful (Dansili), who also won the Gr.2 Rockfel S. as well as the Gr.3 Flame of Tara S., was third in the Gr.1 Matron S. and second in the Grade 1 Belmont Oaks Invitational, and the sire Lost Treasure (War Front), placed in the Gr.3 Mercury S. Wading is a full sister to Bracelet (Montjeu), winner of the Gr.1 Irish Oaks, and a three-parts sister to Athena and Goddess (both by Camelot).

Second dam Cherry Hinton (Green Desert) was second in the Gr.3 Blue Wind S. and is the dam of Bracelet, of Athena, winner of the Grade 1 Belmont Oaks, and of Goddess, winner of the Gr.3 Snow Fairy S. Athena in turn produced Never Ending Story (Dubawi), winner of the Gr.3 Ballylinch Stud 1,000 Guineas Trial S. and the Gr.3 Silver Flash S. and second in the Gr.1 Prix de Diane. Cherry Hinton is a three-parts sister to Sea The Stars (Cape Cross), winner of the Gr.1 Derby and the Gr.1 Prix de l'Arc de Triomphe and an outstanding sire, and a half-sister to Galileo (Sadler's Wells), winner of the Gr.1 Derby and a sire, to Black Sam Bellamy (Sadler's Wells), winner of the Gr.1 Tattersalls Gold Cup and a sire, and to My Typhoon (Giant's Causeway), a Grade 1 winner in the US.

Third dam is the celebrated Urban Sea (Miswaki), winner of the Gr.1 Prix de l'Arc de Triomphe and dam of eight stakes winners, and Cherry Hinton is one of her four daughters. She is a half-sister to King's Best (Kingmambo), winner of the Gr.1 2000 Guineas and a sire.

Galileo, Sea The Stars and Masar (New Approach), winner of the Gr.1 Derby, all appear in Twain's pedigree.

Wading has a colt foal by Frankel.