Nitrogen is the second black-type winner out of Tiffany Case (Uncle Mo), a winner who was Listed-placed. Her half-sister Love to Shop (Violence) won the Listed Toronto Cup and was placed in several graded races, including the Grade 2 Bessarabian Stakes.

Second dam Biblical Point (Point Given) won once and has four winners, including Talk Veuve to Me (Violence), a Grade 3 winner who was placed in the Grade 1 Acorn Stakes.

Third dam Biblical Sense (Blushing Groom) was unraced and produced seven winners, among them the Listed winner Winning Point (Point Given), also placed at Grade 2 level, and the Listed winner Sense Of Duty (Meadowlake).

This female line has also produced Mountain Kingdom, a multiple Group winner in England, and the Grade 1 winner Cool.

Tiffany Case has an unraced 2-year-old filly by Gun Runner named Sniper. Her 2026 foal by Not This Time died, and she was covered again by Not This Time for 2027.